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Product Information
Description |
SAE1 rabbit polyclonal antibody |
Protein full name |
SUMO-activating enzyme subunit 1 |
Synonyms |
Ubiquitin-like 1-activating enzyme E1A, Sae1, SUMO-activating enzyme subunit 1, Uble1a, Aos1, Sua1, N-terminally processed, Ubl1a1 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse SAE1 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Predicted MW. |
39 kDa |
Observed MW. |
39 kDa |
Uniprot ID |
Q9UBE0, Q9R1T2, Q6AXQ0 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
testis |
IHC/IF |
Human, Mouse, Rat |
1: 750-1: 3000 |
brain, breast cancer, colon, colon cancer, lung, small intestine, testis |
Background
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Images
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Western blot analysis of SAE1 (GB111191) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse small intestine using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
Immunohistochemistry of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded mouse lung using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
Immunofluorescence of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens) |
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Immunofluorescence of paraffin embedded rat testis using SAE1 (GB111191) at dilution of 1: 1100 (300x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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