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Product Information
Description |
PCNA rabbit polyclonal antibody |
Protein full name |
Proliferating cell nuclear antigen |
Synonyms |
PCNA, ATLD2, proliferating cell nuclear antigen |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Predicted MW. |
29 kDa |
Observed MW. |
36 kDa |
Uniprot ID |
P12004, P17918, P04961 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
HeLa, SW480, B16, C6, 7721, HCT116 |
IHC/IF |
Human, Mouse, Rat |
1: 200-1: 1000 |
intestinal cancer, liver cancer, lung cancer, ovarian cancer, testis |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
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Western blot analysis of PCNA (GB11010) at dilution of 1: 1000
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Western blot analysis of PCNA (GB11010) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)
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Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)
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Immunofluorescence of paraffin embedded rat testis using PCNA (GB11010) at dilution of 1: 500 (200x lens)
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Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010) at dilution of 1: 500 (200x lens)
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Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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