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Product Information
Description |
GSK3 beta rabbit polyclonal |
Protein full name |
Glycogen synthase kinase-3 beta |
Synonyms |
GSK3B, Gsk3b, 7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3, glycogen synthase kinase 3 beta |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse GSK3β |
Isotype |
IgG |
Predicted MW. |
47 kDa |
Observed MW. |
47 kDa |
Purity |
Affinity purification |
Subcellular location |
Cytoplasm, Nucleus, Cell membrane |
Uniprot ID |
P49841, Q9WV60, P18266 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung |
IHC |
Human, Mouse, Rat |
1: 500-1: 1000 |
testis, ovary cancer |
IF |
Human, Mouse, Rat |
1: 200-1: 500 |
liver cancer |
Background
Glycogen synthase kinase-3 (GSK-3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and an inactivating agent of glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
Images
|
Western blot analysis of GSK3 beta,at dilution of 1: 1000. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: C6 cell lysate Lane 4: Mouse lung tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human ovary cancer GSK3 beta at dilution of 1: 600
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Immunohistochemistry analysis of paraffin-embedded rat testis GSK3 beta at dilution of 1: 600
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Immunofluorescence analysis of paraffin-embedded human liver cancer using GSK3 betaat dilution of 1: 300 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
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