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Product Information
Description |
Hsp47 rabbit polyclonal antibody |
Protein full name |
Serpin H1 |
Synonyms |
47 kDa heat shock protein, Collagen-binding protein, Colligin, Serine protease inhibitor J6, Serpinh1, Cbp1, Hsp47 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Hsp47 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Cytoplasm |
Uniprot ID |
P50454, P19324 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 3000 |
colon cancer, myeloma, ovarian cancer, placenta, skin |
Background
Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.
Images
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Immunohistochemistry of paraffin embedded human colon cancer using HSP47 (GB111425) at dilution of 1:1600 (400x lens) |
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Immunohistochemistry of paraffin embedded human ovarian cancer using HSP47 (GB111425) at dilution of 1:1600 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse myeloma using HSP47 (GB111425) at dilution of 1:1600 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse skin using HSP47 (GB111425) at dilution of 1:1600 (400x lens) |
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Immunohistochemistry of paraffin embedded rat skin using HSP47 (GB111425) at dilution of 1:1600 (400x lens) |
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Immunofluorescence of paraffin embedded human colon cancer using HSP47 (GB111425) at dilution of 1:800 (400x lens) |
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Immunofluorescence of paraffin embedded mouse placenta using HSP47 (GB111425) at dilution of 1:800 (400x lens) |
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Immunofluorescence of paraffin embedded mouse skin using HSP47 (GB111425) at dilution of 1:800 (400x lens) |
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Immunofluorescence of paraffin embedded rat placenta using HSP47 (GB111425) at dilution of 1:800 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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