Anti -Heme-regulated inhibitor Rabbit pAb

價格：在線咨詢

貨號：

KT012032

產(chǎn)品規(guī)格：

100 μL

Applications：

WB/IHC/IF

Species：

HMR/MR/MR

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	Heme-regulated inhibitor rabbit polyclonal
Protein full name	Eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms	Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri
Immunogen	Recombinant protein corresponding to Mouse Heme-regulated inhibitor
Isotype	IgG
Purity	Affinity purification
Predicted MW.	70 kDa
Observed MW.	70 kDa
Subcellular location	Cytoplasm
Uniprot ID	Q9BQI3, Q9Z2R9, Q63185

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	HEK293
IHC	Mouse, Rat	1: 800-1: 1600	bone, lung, inflammation lung
IF	Mouse, Rat	1: 500-1: 1000	bone, lung, inflammation lung

Background

The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.

Images

	Western blot analysis of Eif2ak1 (GB111094) at dilution of 1: 1000 Lane 1: HEK293 cell lysate
	Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

凡合肥萬物生物科技有限公司銷售的產(chǎn)品均有嚴格的質(zhì)量保證。如發(fā)現(xiàn)產(chǎn)品存在包裝規(guī)格疑問，請于收貨之日起七日內(nèi)向我公司總部或當?shù)剞k事處提出，并請保證該產(chǎn)品，以備本公司進行核對檢驗。如果發(fā)現(xiàn)有質(zhì)量問題，請于收貨后一月內(nèi)保留產(chǎn)品50%以上的量，以便本公司回收產(chǎn)品進行質(zhì)檢，確認產(chǎn)品質(zhì)量。如逾期，則視為已經(jīng)對本公司產(chǎn)品進行驗收。聲明：如發(fā)生產(chǎn)品索賠事宜，本公司僅在產(chǎn)品價格范圍內(nèi)酌情賠償，恕不接受超出產(chǎn)品價格范圍之賠償?？蛻粼谑褂眠^程中有任何技術(shù)問題可以撥打技術(shù)售后服務電話我們隨時給予解答。

合肥萬物生物科技有限公司

客服熱線：400-1016-218

QQ：355185756

地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室