服務(wù)熱線
400-1016-218
聯(lián)系方式
- 手機(jī)：400-1016-218
- Q Q：355185756
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室
Anti -Heme-regulated inhibitor Rabbit pAb
價(jià)格：
在線咨詢
貨號：
KT012021
產(chǎn)品規(guī)格：
100ul
Applications：
WB/IHC/IF
Species：
H/M/R,M/R,M/R
購買數(shù)量：
產(chǎn)品詳情
操作說明
注意事項(xiàng)
Product Information
|
Description |
Heme-regulated inhibitor rabbit polyclonal |
|
Protein full name |
Eukaryotic translation initiation factor 2-alpha kinase 1 |
|
Synonyms |
Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase, Eif2ak1, Heme-controlled repressor, HCR, Heme-regulated inhibitor, Hri |
|
Immunogen |
Recombinant protein corresponding to Mouse Heme-regulated inhibitor |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
70 kDa |
|
Observed MW. |
70 kDa |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
HEK293 |
|
IHC |
Mouse, Rat |
1: 800-1: 1600 |
bone, lung, inflammation lung |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
bone, lung, inflammation lung |
Background
The Eif2ak1 Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells, controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties.
Images
|
|
Western blot analysis of Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse bone using Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse inflammation lung using Eif2ak1 (GB111094) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
凡合肥萬物生物科技有限公司銷售的產(chǎn)品均有嚴(yán)格的質(zhì)量保證。如發(fā)現(xiàn)產(chǎn)品存在包裝規(guī)格疑問，請于收貨之日起七日內(nèi)向我公司總部或當(dāng)?shù)剞k事處提出，并請保證該產(chǎn)品，以備本公司進(jìn)行核對檢驗(yàn)。如果發(fā)現(xiàn)有質(zhì)量問題，請于收貨后一月內(nèi)保留產(chǎn)品50%以上的量，以便本公司回收產(chǎn)品進(jìn)行質(zhì)檢，確認(rèn)產(chǎn)品質(zhì)量。如逾期，則視為已經(jīng)對本公司產(chǎn)品進(jìn)行驗(yàn)收。 聲明：如發(fā)生產(chǎn)品索賠事宜，本公司僅在產(chǎn)品價(jià)格范圍內(nèi)酌情賠償，恕不接受超出產(chǎn)品價(jià)格范圍之賠償?？蛻粼谑褂眠^程中有任何技術(shù)問題可以撥打技術(shù)售后服務(wù)電話我們隨時(shí)給予解答。
合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室