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Anti -Cytokeratin 5 Rabbit pAb

價格: 在線咨詢
貨號:
KT011907
產品規(guī)格:
100ul
Applications:
WB/IHC/IF
Species:
H/M,H/M/R,H/M/R
購買數(shù)量:
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產品詳情 操作說明 注意事項

Product Information


Description

Cytokeratin 5 rabbit polyclonal

Protein full name

Keratin, type II cytoskeletal 5

Synonyms

Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, Krt5, Krt2-5

Immunogen

Recombinant protein corresponding to Mouse Cytokeratin 5

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell Membrane

Predicted MW.

62 kDa

Observed MW.

62 kDa

Uniprot ID

P13647Q922U2Q6P6Q2




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1: 500-1: 1000

skin

IHC

Human, Mouse, Rat

1: 1000-1: 2000

breast cancer, skin, bladder

IF

Human, Mouse, Rat

1: 1000-1: 3000

ovary, breast, skin, esophagus, bladder




Background


The protein encoded by this gene is a member of the keratin gene family. It is one type II keratin, is coexpressed with keratin 14 in stratified squamous epithelia. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex.




Images


Western blot analysis of Cytokeratin 5 (GB111246) at dilution of 1: 1000
Lane 1: A431 cell lysate
Lane 2: Mouse skin tissue lysate

Immunohistochemistry analysis of paraffin-embedded human breast cancer using CK5 (GB111246) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse skin using CK5 (GB111246) at dilution of 1: 2000

image.png

Immunohistochemistry analysis of paraffin-embedded rat skin using CK5 (GB111246) at dilution of 1: 2000

image.png

Immunohistochemistry analysis of paraffin-embedded rat bladder using CK5 (GB111246) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed human ovary using CK5 (GB111246) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed human breast using CK5 (GB111246) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed mouse skin using CK5 (GB111246) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed rat esophagus using CK5 (GB111246) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed rat bladder using CK5 (GB111246) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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