TUBB3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, tubulin beta 3 class III, β3- tubulin
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse β3- tubulin
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cytoplasm
Predicted MW.
55 kDa
Observed MW.
55 kDa
Uniprot ID
Q9ERD7, Q4QRB4
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1:1000-1:2000
cerebral cortex, hippocampus, brain
ICC/IF
Mouse
1: 500-1: 1000
Neuro-2a
Background
Cysteine residues in class III β-tubulin are actively involved in regulating ligand interactions and microtubule formation. Proteomic analysis has revealed that many factors bound to these cysteine residues are involved in the oxidative stress and glucose deprivation response.This is particularly interesting in light of the fact that class III β-tubulin first appears in the phylogenetic tree when life emerged from the seas and cells were exposed to atmospheric oxygen.
Images
Western blot analysis of β-III-Tubulin (GB12139) at dilution of 1: 1000
Immunofluorescence analysis of mouse Neuro-2a cells using β3- tubulin (GB12139)
at dilution of 1: 1000
A: DAPI nuclear stain ( blue )
B: Anti-β3- tubulin ( red )
C: Merge
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .