Anti -Vimentin Mouse mAb

價格：在線咨詢

貨號：

KT011582

產品規(guī)格：

100 μL

Applications：

WB | IHC | IF

Species：

H,M,R | M,R

品牌：

萬物

購買數量：

立即詢價在線咨詢 400-1016-218

產品詳情操作說明注意事項

Product Information

Description	Vimentin mouse monoclonal antibody
Protein full name	Vimentin
Synonyms	VIM, CTRCT30, HEL113, vimentin
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse vimentin
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm
Predicted MW.	57 kDa
Observed MW.	57 kDa
Uniprot ID	P08670, P20152, P31000

Applications

Applications	Species	Dilution	Positive Sample
WB	Human, Mouse, Rat	1: 1000-1: 2000	lung
IHC/IF	Mouse, Rat	1: 500-1: 1000	colon, thymus, tonsil

Background

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.

Images

	Western blot analysis of Vimentin (GB12192) at dilution of 1: 1000
	Immunohistochemistry of paraffin embedded human tonsil using vimentin (GB12192) at dilution of 1: 1000 (400x lens)
	Immunofluorescence of paraffin embedded mouse colon using vimentin (GB12192) at dilution of 1: 500 (400x lens)
	Immunofluorescence of paraffin embedded mouse thymus using vimentin (GB12192) at dilution of 1: 500 (400x lens)

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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