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Product Information
Description |
Lck rabbit polyclonal |
Protein full name |
Proto-oncogene tyrosine-protein kinase LCK |
Synonyms |
Leukocyte C-terminal Src kinase, |
Immunogen |
Recombinant protein corresponding to Mouse Lck |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
58 kDa |
Observed MW. |
58 kDa |
Subcellular location |
|
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
thymus, lymph nodes, spleen |
IHC |
Mouse, Rat |
1: 1500-1: 4000 |
lung, lymph, spleen, thymus |
IF |
Mouse, Rat |
1: 1500-1: 3000 |
lung, lymph, spleen |
Background
LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.
Images
Western blot analysis of Lck (GB111091) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: Mouse thymus tissue lysate Lane 3: Mouse lymph nodes tissue lysate Lane 4: Mouse spleen tissue lysate Lane 5: Rat thymus tissue lysate Lane 6: Rat lymph nodes tissue lysate Lane 7: Rat spleen tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded rat lymph using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded mouse thymus using Lck (GB111091) at dilution of 1: 1900 | |
Immunohistochemistry analysis of paraffin-embedded rat thymus using Lck (GB111091) at dilution of 1: 1900 | |
Immunofluorescent analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900 | |
Immunofluorescent analysis of paraffin-embedded rat lung using Lck (GB111091) at dilution of 1: 1900 | |
Immunofluorescent analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900 | |
Immunofluorescent analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 3000 | |
Immunofluorescent analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 3000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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