Anti -GAPDH Mouse mAb

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貨號：

產(chǎn)品規(guī)格：

100 μL

Applications：

WB | IHC | IF

Species：

H,M,R | H,M,R | H,M,R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	GAPDH mouse monoclonal
Protein full name	Glyceraldehyde-3-phosphate dehydrogenase
Synonyms	GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Isotype	IgG
Predicted MW.	37 kDa
Observed MW.	37 kDa
Subcellular location	Cytoplasm, Nucleus, Membrane
Purity	Affinity purification
Uniprot ID	P04406,P04797,P16858

Applications

applications	species	dilution	Positive tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	spleen, lung, kidney, brain, heart, skeletal muscle
IHC	Human, Mouse, Rat	1: 200-1: 400	stomach, testis, kidney
IF	Human, Mouse, Rat	1: 200-1: 400	stomach, testis, kidney

Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.

Images

	Western blot analysis of GAPDH (GB12002) at dilution of 1:1000
	Western blot analysis of GAPDH (GB12002) at dilution of 1:1000
	Immunohistochemistry analysis of paraffin-embedded human stomach using GAPDH (GB12002) at dilution of 1:200
	Immunohistochemistry analysis of paraffin-embedded mouse testis using GAPDH (GB12002) at dilution of 1:200
	Immunohistochemistry analysis of paraffin-embedded rat kidney using GAPDH (GB12002) at dilution of 1:200

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

Publications:

	Title	Journal	Pubmed ID	Application
1	Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells.	Aging (Albany NY)	33290258	WB
2	Hyperoside Attenuates Bleomycin-Induced Pulmonary Fibrosis Development in Mice. Abstract	Frontiers in Pharmacology	33192501	WB
3	African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.	Virologica Sinica	33141406	WB
4	Bioinformatics analysis of LINC01554 and its co?expressed genes in hepatocellular carcinoma.	Oncology Reports	33000250	WB
5	DEPDC1B promotes migration and invasion in pancreatic ductal adenocarcinoma by activating the Akt/GSK3β/Snail pathway.	Oncology Letters	32934714	WB
6	The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29.	Scientific Reports	33004906	WB
7	Protective effect of dimethyl fumarate on oxidative damage and signaling in cardiomyocytes.	Molecular Medicine Reports	32945364	WB
8	RNF181 modulates Hippo signaling and triple negative breast cancer progression.	Cancer Cell International	32655323	WB
9	A protein palmitoylation cascade regulates microtubule cytoskeleton integrity in Plasmodium.	The EMBO Journal	32395856	WB
10	Hypoxia/reoxygenation activates the JNK pathway and accelerates synovial senescence.	Molecular Medicine Reports	32377698	WB

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