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Anti -GAPDH Rabbit pAb

價(jià)格: 在線咨詢
貨號:
DRF10110
產(chǎn)品規(guī)格:
100 μL
Applications:
WB
Species:
H,M,R
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

GAPDH rabbit polyclonal

Protein full name

Glyceraldehyde-3-phosphate dehydrogenase

Synonyms

GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse GAPDH

Isotype

IgG

Predicted MW.

37kDa

Observed MW.

37kDa

Purity

Affinity purification

Uniprot ID

P04406,P04797,P16858



Applications



applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1:1000-1:2000

brain, spleen, kidney, liver




Background


Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.




Images



圖片 2.png

Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: MCF-7 cell lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse kidney tissue lysate

Lane 6: Mouse brain tissue lysate

Lane 7: Rat liver tissue lysate

Lane 8: Rat kidney tissue lysate

Lane 9: Rat brain tissue lysate

圖片 3.png

Western blot analysis of GAPDH (GB11002) at dilution of 1: 2000

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: Mouse brain tissue lysate

Lane 5: Mouse spleen tissue lysate

Lane 6: Rat kidney tissue lysate

Lane 7: Rat brain tissue lysate




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .



Publications:


Title

Journal

Pubmed ID

Application

1

Resveratrol alleviates oxygen/glucose deprivation/reoxygenation-induced neuronal damage through induction of mitophagy.

Molecular Medicine Reports

33236158

WB

2

Deubiquitinase USP7 regulates Drosophila aging through ubiquitination and autophagy.

Aging (Albany NY)

33221768

WB

3

Epigallocatechin gallate induces chemopreventive effects on rats with diethylnitrosamine-induced liver cancer via inhibition of cell division cycle 25A.

Molecular Medicine Reports

33000276

WB

4

Xiaoyao Pills Attenuate Inflammation and Nerve Injury Induced by Lipopolysaccharide in Hippocampal Neurons In Vitro.

Neural Plasticity

33014035

WB

5

PEDF promotes the repair of bone marrow endothelial cell injury and accelerates hematopoietic reconstruction after bone marrow transplantation.

Journal of Biomedical Science

32873283

WB

6

Telomerase inhibition decreases esophageal squamous carcinoma cell migration and invasion.

Oncology Letters

32782603

WB

7

Dihydrolipoic acid protects against lipopolysaccharide-induced behavioral deficits and neuroinflammation via regulation of Nrf2/HO-1/NLRP3 signaling in rat.

Journal of Neuroinflammation

32450903

WB

8

5-Aza-2'-deoxycytidine advances the epithelial-mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements.

Journal of Cell Science

32193333

WB

9

Effect of cold stress on ovarian & uterine microcirculation in rats and the role of endothelin system.

Reproductive Biology and Endocrinology : RB&E

32290862

WB

10

Xiaoyao Pills Prevent Lipopolysaccharide-Induced Depression by Inhibiting Inflammation and Protecting Nerves.

Frontiers in Pharmacology

31798446

WB


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