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Product Information
Description |
SARS-CoV-2 nucleocapsid protein rabbit polyclonal |
Protein full name |
SARS-COV-2 nucleocapsid protein |
Synonyms |
2019-nCoV nucleocapsid protein, NC, Nucleoprotein Protein N , Covid-19 |
Immunogen |
recombinant SARS-CoV-2 nucleocapsid protein expressed in 293F cells. |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
50 kDa |
Observed MW. |
50 kDa |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
----------- |
1:1000-1:5000 |
Use at an assay dependent sample |
Background
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
Images
Western blot analysis of SARS-CoV-2 nucleocapsid protein (GB112133) at dilution of 1:1000. Lane 1-4: recombination RBD protein expressed in 293F cell at different concentration gradient. |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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