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Product Information
Description | beta Tubulin mouse monoclonal |
Protein full name |
Tubulin beta chain |
Synonyms |
TUBB, CDCBM6, M40, OK/SW-cl.56, TUBB1, TUBB5, CSCSC1, tubulin beta class I,Tubulin Beta-5 Chain |
Immunogen |
KLH conjugated Synthetic peptide corresponding to human β- tubulin |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. |
55 kDa |
Observed MW. |
55 kDa |
Subcellular location |
Cytoplasm |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 2000-1: 5000 |
Brain,lung,Hela,HepG2,A549 |
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
Kidney,liver cancer,esophageal cancer |
IF |
Human, Mouse, Rat |
1: 1000-1: 2000 |
Kidney,liver cancer,esophageal cancer |
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Images
Western blot analysis of β- tubulin (GB13017-2) at dilution of 1:2000.
Lane 1:Hela cell lysate Lane 2:HepG2 cell lysate Lane 3:A549 cell lysate Lane 4:CNE2 cell lysate Lane 5:Jurkat cell lysate Lane 6:NIH/3T3 cell lysate Lane 7:Raw264.7 cell lysate Lane 8:H9C2 cell lysate
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Immunohistochemistry analysis of paraffin-embedded rat brain using β- tubulin (GB13017-2) at dilution of 1:1000 |
Immunofluorescence analysis of paraffin-embedded human esophageal cancer using β- tubulin (GB13017-2) at dilution of 1:1000
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Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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