Anti -EGFP Mouse mAb

價(jià)格：在線咨詢

貨號(hào)：

DRF10134

產(chǎn)品規(guī)格：

100 μL

Applications：

WB | IF

Species：

tag/tag

品牌：

萬物

購買數(shù)量：

立即詢價(jià) 在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	EGFP mouse monoclonal
Protein full name	Enhanced GFP
Synonyms	Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP
Immunogen	Recombinant protein corresponding to Mouse EGFP
Isotype	IgG
Purity	Affinity purification
Predicted MW.	27 kDa
Observed MW.	29 kDa

Applications

Applications	Species	Dilution	Positive Tissue
WB	tag	1: 500-1: 1000	cells/ tissues with eGFP
IF	tag	1: 2000-1: 4000	CHO-S

Background

Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.

Images

Western blot analysis of eGFP (GB12602)

at dilution of 1: 1000

Lane 1: 293T cell lysate

Lane 2: 293T with eGFP cell lysate

Lane 3: NIH/3T3 cell lysate

Lane 4: NIH/3T3 with eGFP cell lysate

圖片 3.png A

Immunofluorescent analysis of transfect CHO-S cells using eGFP (GB12602) at dilution of 1: 2000

A: DAPI nuclear stain ( blue )

B: Anti-eGFP ( red ）

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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